I don't even know where should I continue with this blog. I've been absent here for a long time and these absences add up. So I will attempt to go with this blog as far as I can. Of course I draw a lot but I don't always have the time and energy to write these entries. But really. I WILL TRY.
By the time Evelyne arrived to Budapest. The girl from America, with a British father and a Russian mother. Obviously she's Jewish and me and my family are trying to help her as much as we can because she came here to study at Semmelweis University.
On the first day of this procedure we've pipetted together several liquids which were essential to perform the cloning. These were (at least what I remember) DNA fragments, nuclease free water, linearized plasmid, ligase, puffer, Xgal, E. Coli and something else I don' really remember. We pipetted, melted, shook, knuckled and incubated them until they were ready for proliferation. Then we've waited.
When the project have started first we discussed the topic. The identification of Proteus Anguinus (cave salamander) from environmental DNA. They had to prove that the DNA in the water sample is really from this species. Therefore they had to sequence a gene specific for PA. But this DNA fragment is too short for automated Sanger-sequencing so they had to use a cloning vector to isolate, amplify and lengthen this fragment to make it applicable for sequencing.
A eukaryotic cell and DNA transcription and copying.
So I went to the laboratory and Orsi showed me every machine and technique they use for research. She also gave me some books on NGS (New Generation Sequencing), anatomy and told me about a book on history of science.